The paper introduces 3D-4i imaging and analysis to study cell cycle dynamics and transcriptional output during zygotic genome activation in zebrafish embryos.

Nature Communications · Sep 17, 2026Peer-reviewedSystems Biology

Shield-4i: A Whole-mount Multiplexed Imaging Platform for Studying Multiscale Information Flow in 3D Multicellular Systems

Ruth Hornbachner, Shayan Shamipour, Feyza Nur Arslan, Rui Fan, Max Hess (17838936), Florian Curvaia, Joel Lüthi, Andrew C. Oates, Ivan Bedzhov, Darren T. Gilmour, Virginie Uhlmann, Lucas Pelkmans

Shield-4i enables high-throughput, subcellular-resolution imaging of whole-mount structures to study multiscale information flow in multicellular systems.

bioRxiv · Jul 2, 2026 · PreprintSystems Biology

Yolk granule fusion and microtubule aster formation regulate cortical granule translocation and exocytosis in zebrafish oocytes

Shayan Shamipour, Laura Hofmann, Irene Steccari, Roland Kardos, Carl‐Philipp Heisenberg

The paper shows that yolk granule fusion and microtubule aster formation drive cortical granule translocation and exocytosis in zebrafish oocytes during maturation.

PLoS Biology · Jun 8, 2023Peer-reviewedDevelopmental Biology