Darren T. Gilmour

This paper introduces a chemigenetic biosensor, WHaloEKAR, that reports ERK activity through fluorescence lifetime changes, enabling spatially resolved and multiplexed measurements in cultured cells and live animals.

The paper introduces 3D-4i imaging and analysis to study cell cycle dynamics and transcriptional output during zygotic genome activation in zebrafish embryos.

Shield-4i enables high-throughput, subcellular-resolution imaging of whole-mount structures to study multiscale information flow in multicellular systems.

The paper shows that Fgf3 in zebrafish lateral line organogenesis represses target genes through nuclear accumulation, distinct from its extracellular signaling role.

The paper introduces nuQLOUD, a framework to analyze tissue architecture, revealing two archetypes in zebrafish embryos driven by cadherin expression.

The paper identifies conserved paralog substitutions during neuronal differentiation across species and shows their impact on neuron development.

The paper develops a point cloud-based algorithm to analyze 3D microscopy data of zebrafish tissue development, integrating morphology, gene expression, and contextual knowledge to uncover cellular architecture patterns.