Beyond Static Screens: optical pooled screening of signaling dynamics using time-lapse FLIM
Sravasti Mukherjee, Menno van Tooren, Dimitris Sfakianakis, Dominique Kemps, Jeffrey B. Klarenbeek, Giulia Zanetti, Hendrik J. Kuiken, Cor Lieftink, Bram van den Broek, Roderick L. Beijersbergen, Kees Jalink
The paper develops a high-throughput live-cell screening platform using time-lapse FRET-FLIM to study dynamic signaling phenotypes, demonstrating its effectiveness in identifying regulators of cAMP signaling.
bioRxiv · Jul 11, 2025 · PreprintCell Biology