Author

Manabu Shirai

National Cerebral and Cardiovascular CenterORCID 0000-0002-1063-5822Unclaimed ORCID profile · follow to get notified of new preprints

Biology Disciplines

Click to filter author papers

Cell Biology· 1 Developmental Biology· 1 Neuroscience· 1

Signature Assays, Organisms & Methods

Click to filter · or open tag

Research Focus:differential expression· 3 Organism:mouse· 3 Assay:bulk RNA-seq· 2 Research Focus:cell type annotation· 2 Approach:experimental assay· 2 Research Focus:cell-cell communication· 1 Research Focus:clustering· 1 Research Focus:gene regulatory network inference· 1

Frequent Co-Authors

Noriaki SasaiiD· 2 shared Agnes Lee Chen OngiD· 1 shared Chiemi Yamashiro· 1 shared Fumiaki HigashijimaiD· 1 shared Fumiyasu SeikeiD· 1 shared Jumpei Ito· 1 shared Jun AonoiD· 1 shared Katsuji InoueiD· 1 shared

Activity by Year

2020-2025 · peak 1/yr

Co-Author Collaboration Network

10 top collaborators · 17 mutual co-author links

Click any collaborator node to inspect or filter shared papers

Focal author (center)Selected co-authorORCID co-authorUnlinked name

Curved chords = mutual co-authorship

2 shared papersDevelopmental Biology

Noriaki SasaiORCID

Mutual co-authors in cluster

Author profile →

3 papers

Recent·Most cited

Osteochondrogenic Transdifferentiation of Vascular Smooth Muscle Cells and Microenvironmental Dynamics in Medial Arterial Calcification

Yasuhisa Nakao, Tomohisa Sakaue, Jumpei Ito, Mika Hamaguchi, Kayo Takahashi, Masayoshi Kukida, Fumiyasu Seike, Jun Aono, Shigemiki Omiya, Yuji Sakahashi, Morikatsu Yoshida, Masaki Wakabayashi, Manabu Shirai, Kazunori Yasuda, Katsuji Inoue, , Osamu Yamaguchi

This paper establishes an OTAC mouse model to study medial arterial calcification, identifying osteochondrogenic transdifferentiation of vascular smooth muscle cells and associated microenvironmental changes using scRNA-seq.

bioRxiv · Oct 28, 2025 · PreprintCell Biology

Hedgehog Activity Gradient in Combination with Transcription Network Confers Multiple Hypothalamic Identities

Maho Yamamoto, Agnes Lee Chen Ong, Takuma Shinozuka, Manabu Shirai, Noriaki Sasai

The paper investigates how Hedgehog signaling and transcription factors regulate hypothalamic regionalization during development.

bioRxiv · Aug 23, 2022 · PreprintDevelopmental Biology

Light-dependent induction of Edn2 expression and attenuation of retinal pathology by endothelin receptor antagonists in Prominin-1- deficient mice

Yuka Kobayashi, Shizuka Watanabe, Manabu Shirai, Chiemi Yamashiro, Tadahiko Ogata, Fumiaki Higashijima, Takuya Yoshimoto, Takahide Hayano, Yoshiyuki Asai, Noriaki Sasai, Kazuhiro Kimura

This study identifies Edn2 upregulation in Prom1-deficient mice and shows that endothelin receptor antagonists reduce retinal degeneration.

bioRxiv · Dec 7, 2020 · PreprintNeuroscience