Synthetic BiologybioRxivPreprint
Improving cell-free expression of membrane proteins by tuning ribosome co-translational membrane association and nascent chain aggregation
Jan Steinkühler, Justin A. Peruzzi, Antje Krüger…·Neha P. Kamat
This paper improves cell-free expression of membrane proteins by identifying factors affecting synthesis and developing a model to optimize yield through membrane composition and N-terminal domain sequence adjustments.