Antje Krüger

Scalable cell-free production of active T7 RNA polymerase

This paper demonstrates scalable cell-free production of active T7 RNA polymerase with high purity and activity, suitable for decentralized biotherapeutic manufacturing.

Community science designed ribosomes with beneficial phenotypes

This study uses community science and experimental screening to design mutant ribosomes with improved protein synthesis and cell growth in vitro and vivo.

Improving cell-free expression of membrane proteins by tuning ribosome co-translational membrane association and nascent chain aggregation

This paper improves cell-free expression of membrane proteins by identifying factors affecting synthesis and developing a model to optimize yield through membrane composition and N-terminal domain sequence adjustments.

Multivalent designed proteins protect against SARS-CoV-2 variants of concern

The paper designs and characterizes trivalent miniprotein binders that neutralize SARS-CoV-2 variants with low pM IC50 values and provide prophylactic and therapeutic protection in mice.

A high-throughput, automated, cell-free expression and screening platform for antibody discovery

This paper develops an automated cell-free platform for rapid antibody discovery and screening, demonstrating its effectiveness with SARS-CoV-2 neutralizing antibodies.

Development of a clostridia-based cell-free system for prototyping genetic parts and metabolic pathways

This paper develops a high-yielding cell-free gene expression system from Clostridium autoethanogenum to enable in vitro prototyping of genetic parts and metabolic pathways.

Cell-free styrene biosynthesis at high titers

This paper develops a cell-free system for styrene biosynthesis, achieving titers 10 times higher than cellular methods by optimizing reaction conditions.