Han Wang

TAG-IN enables efficient, flexible, and scalable endogenous gene tagging in C. elegans by using CRISPR to create seed alleles and Flp-mediated cassette exchange for tag swapping.

This study identifies affl-2, a C. elegans ortholog of the human AF4/FMR2 family, as a regulator of heat shock induced gene expression.

This paper describes a CRISPR/Cas9-based method to generate putative null mutants in C. elegans by inserting a STOP-IN cassette, achieving a 46% insertion rate and identifying phenotypes for several genes.