Abigail Isaacson

This paper develops a protocol to generate human iPSC-derived pancreatic organoids with proper architecture and patterning, aiming to replace animal models in studying pancreas development and disease.

The paper studies a heterozygous missense variant in GLI2 in human iPSCs and finds it impairs endocrine pancreas development and predisposes to diabetes.

The paper uses single-cell RNA-seq of differentiating iPS cells from 125 donors to identify expression quantitative trait loci influencing gene expression during endoderm differentiation.