Juan Pablo Tosar

The paper describes a method to isolate and characterize lipoprotein classes in plasma, revealing unique lipidomic, proteomic, and small RNA signatures for each class.

The paper presents a modified immuno-TEM protocol to analyze tetraspanin positivity in EVs and shows that CD9 and CD81 are more enriched in small EVs.

The paper investigates the correlation between loss of microRNAs, protein-coding genes, and morphological complexity in syndermatan invertebrates, finding a strong association in regressive evolution.

This study shows that naked extracellular RNA can be pro-inflammatory and functionally active in immune cells when ribonuclease inhibitors are present, challenging the need for RNA encapsulation.

This study shows PIWIL1 localizes to centrosomes during mitosis in CRC cells, affecting cell cycle progression and suggesting a role in stem cell maintenance.

SPIRFISH detects protein and RNA in single nanoparticles, revealing variability in structure and RNA packaging efficiency.

The paper identifies nicked tRNAs as stable extracellular RNA species in biofluids and demonstrates methods to recover full-length tRNAs from these nicked forms.

The paper shows miR-21-5p and miR-30a-5p are identical between human and bovine, and their presence in human blood after milk ingestion is likely due to endogenous regulation or technical issues, not xenomiR uptake.