Kenneth Whitaker Witwer

This paper provides a standardized protocol for isolating and characterizing extracellular vesicles from mouse retinal tissue, improving the study of in vivo EV biology.

This study engineers extracellular vesicles with Syncytin-1 to alter their biodistribution and shows improved persistence and organ targeting in non-human primates.

The paper identifies and validates novel neuronal EV surface markers, CNTNAP2 and STX1B, for Alzheimer's disease biomarker discovery through proteomic analysis of EVs from hiPSC-derived neurons and human brain.

This study develops magnetically labeled iPSC-derived extracellular vesicles for MRI/MPI-guided myocardial infarction therapy, showing improved cardiac function and detectability.

The paper describes a method to isolate and characterize lipoprotein classes in plasma, revealing unique lipidomic, proteomic, and small RNA signatures for each class.

The paper presents a modified immuno-TEM protocol to analyze tetraspanin positivity in EVs and shows that CD9 and CD81 are more enriched in small EVs.

The paper identifies factors influencing RNA sorting into extracellular vesicles, finding size and structuredness significantly affect enrichment of circular and linear RNAs.

The paper analyzes extracellular vesicles from MS and control white matter to identify pathway dysregulation and cellular origin shifts associated with the disease.

This study shows that naked extracellular RNA can be pro-inflammatory and functionally active in immune cells when ribonuclease inhibitors are present, challenging the need for RNA encapsulation.

This study investigates how extracellular vesicles adhere to different surfaces and shows that borosilicate glass coverslips can effectively immobilize EVs for imaging techniques.

The paper investigates how extracellular vesicles interact with B cells in nonhuman primates and humans, showing uptake via endocytosis and membrane fusion, and highlights potential strategies to modulate EV-B cell interactions.

SPIRFISH detects protein and RNA in single nanoparticles, revealing variability in structure and RNA packaging efficiency.

The paper shows that EV miRNAs from WT mice improve sociability in immunodeficient mice by modulating synaptic function and immune-neuron communication.

The paper analyzes small RNA-seq data from SARS-CoV-1 and SARS-CoV-2 infected cells to identify novel viral short RNAs and their potential roles in infection.

The paper characterizes extracellular vesicles (EVs) from different brain regions, revealing regional differences in EV yield, surface markers, and RNA content.

The paper profiles RNA in brain tissue and derived extracellular vesicles from SIV-infected macaques to identify dysregulated RNAs and their potential roles in CNS pathology.

The paper identifies nicked tRNAs as stable extracellular RNA species in biofluids and demonstrates methods to recover full-length tRNAs from these nicked forms.

The paper identifies differentially enriched proteins in brain-derived extracellular vesicles from Parkinson's disease, PSP, and control samples using surface phenotyping and proteomics.

This study profiles miRNAs in primate cervicovaginal lavage and EVs during the menstrual cycle and SIV infection, identifying miR-186-5p as a potential retroviral restriction factor in macrophages.

This study evaluates the impact of species and processing parameters on the recovery and content of brain tissue-derived extracellular vesicles using modified protocols.

The paper investigates the distribution of miR-21 between HMW-RISC and LMW-RISC fractions in thyroid cancer cell lines and finds miR-21 is more enriched in LMW-RISC.

The paper shows miR-21-5p and miR-30a-5p are identical between human and bovine, and their presence in human blood after milk ingestion is likely due to endogenous regulation or technical issues, not xenomiR uptake.

This study confirms the stability of miRNAs in platelet-poor plasma and purified RNA, showing they are less prone to degradation than other RNAs.

The paper shows that plant miRNAs detected in human plasma are likely artifacts, not evidence of cross-kingdom RNAi.

This study shows that serum extracellular vesicle depletion affects HIV-1 production and infectivity in cell cultures, with potential confounding effects from other particles.

The paper tests whether synthetic miR-16-5p acts as a reverse transcriptase co-factor to improve detection of low-abundance miRNAs and finds no support for this role.