Marco Li Calzi

The paper presents a modified immuno-TEM protocol to analyze tetraspanin positivity in EVs and shows that CD9 and CD81 are more enriched in small EVs.

This study shows that naked extracellular RNA can be pro-inflammatory and functionally active in immune cells when ribonuclease inhibitors are present, challenging the need for RNA encapsulation.

The paper identifies nicked tRNAs as stable extracellular RNA species in biofluids and demonstrates methods to recover full-length tRNAs from these nicked forms.