The two crystal structures of unloaded and cargo-loaded Vacuolar Sorting Receptor 1 lumenal domain (VSR1-Nt) reveal two cargo binding sites and a mechanism of transport.
Rafael J. Borges, Mahmoud K. Eldahshoury, Rebecca J. Parr, Marilyn Paul, Paul K. Fyfe, Joanne E. Nettleship, Jing An, Nita R. Shah, Ana J Loureiro Levada, Marcos R.M. Fontes, Raymond J. Owens, Jürgen Denecke, Andrew Quigley, Adrian Goldman, Vincent L. G. Postis, , Carine De Marcos Lousa
The paper determines the crystal structures of unloaded and cargo-loaded VSR1-Nt, revealing two cargo binding sites and a calcium-dependent mechanism for cargo binding and release.
bioRxiv · Dec 7, 2024 · PreprintPlant Biology