This study uses single-cell multiomics to resolve the developmental and regulatory logic of Cannabis sativa glandular trichomes, identifying new cell types and regulatory programs.

bioRxiv · May 18, 2026 · PreprintPlant Biology

Mitochondrial Retrograde Signaling in Arabidopsis thaliana: heterogenous, spatial and polarised aspects

Yanqiao Zhu, Minxuan Li, Minxuan Li, Rijad Sarić, Feiran Han, Xinxin Zhou, Xiachao Wang, Yuhua Liu, Chi Zhang, Huixia Shou, Ghazanfar Abbas Khan, Mathew G. Lewsey, James Whelan

This study uses single-nucleus and spatial RNA sequencing to reveal heterogeneous, spatial, and polarized mitochondrial stress signaling in Arabidopsis thaliana, showing how mitochondrial dysfunction reshapes cell identity trajectories.

bioRxiv · Feb 10, 2026 · PreprintPlant Biology

Antigen-dependent activation of marginal zone B cells amplifies hypertension

Hericka Bruna Figueiredo Galvão, Maria Jelinic, Maggie Lieu, Vivian Tran, Buddhila Wickramasinghe, Jake N. Robertson, Tayla A. Gibson Hughes, Henry Diep, Asha Haslem, Mathew G. Lewsey, Alexander Bobik, Christopher Graeme Sobey, Tomasz Jan Guzik, Grant Raymond Drummond, Antony Vinh

This study identifies marginal zone B cells as antigen-responsive subsets that amplify immune responses in hypertension through clonal expansion and intercellular communication with CD8+ T cells.

bioRxiv · Oct 17, 2025 · PreprintPhysiology

High-resolution transcriptomic profiling of the aortic cellular landscape during hypertension reveals novel drivers of vascular fibrosis

Maria Jelinic, Malathi S.I. Dona, Tayla A. Gibson Hughes, Gabriella E. Farrugia, Rebecca L. Harper, Ian Hsu, Asha Haslem, Vivian Tran, Hericka Bruna Figueiredo Galvão, Henry Diep, Quynh Nhu Dinh, Taylah L. Gaynor, Tomasz Jan Guzik, Matteo Lemoli, Christopher Graeme Sobey, , Grant Raymond Drummond

This study identifies a novel fibroblast subcluster, Fibro-Cthrc1, as a driver of aortic fibrosis in hypertension using scRNA-seq and validates its association with blood pressure in humans.

bioRxiv · Mar 13, 2025 · PreprintPhysiology

Towards accurate, reference-free differential expression: A comprehensive evaluation of long-read de novo transcriptome assembly

Feng Yan, Pedro L. Baldoni, James Lancaster, Matthew E. Ritchie, Mathew G. Lewsey, Quentin A. Gouil, N. Davidson

This study evaluates long-read de novo transcriptome assembly tools for differential expression analysis without a reference genome, finding RNA-Bloom2 with Corset as the most accurate and efficient.

bioRxiv · Feb 2, 2025 · PreprintBioinformatics

Characterization of the Cannabis sativa glandular trichome epigenome

Lee James Conneely, Bhavna Hurgobin, Sophia Ng, Muluneh Tamiru, Mathew G. Lewsey

This study maps histone modifications and integrates transcriptomics in Cannabis sativa glandular trichomes to link chromatin states to specialized metabolism gene expression.

BMC Plant Biology · Nov 14, 2024Peer-reviewedPlant Biology

AgriSeqDB: an online RNA-Seq database for functional studies in agriculturally relevant plant species

Andrew J. Robinson, Muluneh Tamiru, Rachel Salby, Clayton Bolitho, Andrew Williams, Simon Huggard, Eva Fisch, Kathryn Unsworth, James Whelan, Mathew G. Lewsey

AgriSeqDB provides a user-friendly RNA-Seq database for visualizing and analyzing transcriptome data from agricultural crops and Arabidopsis.

BMC Plant Biology · Sep 19, 2018Peer-reviewedGenomics