Natalio Krasnogor

This paper develops a computational method to select DNA origami scaffold sequences that minimize off-target interactions, validated through AFM and optical tweezers experiments.

This paper introduces an algorithm to convert raw DNA origami sequences back into schematic designs, enabling easier modification and reuse of existing nanostructures.

OptDesign identifies optimal strain design strategies for biochemical production in E. coli by combining regulation and knockout interventions using a two-step approach.

The paper introduces a version control system for cell engineering that combines cloud-based software with molecular barcoding to enhance transparency and traceability.

The paper demonstrates the use of Targetron technology with CRISPR-aided counterselection to efficiently deliver exogenous DNA sequences into Pseudomonas putida.

This paper demonstrates the in vitro synthesis of scaffolded RNA origami using a single-pot transcription reaction and validates its structure through imaging and simulation.

The paper introduces a digital twinning system to link engineered cells to their digital history, enabling version control for collaborative strain engineering.

Easybiotics provides a graphical user interface for Simbiotics, enabling biologists without programming skills to design, simulate, and analyze 3D bacterial population models.

The paper generates patient-specific retinal organoids and RPE from RP type 11 patients to show PRPF31 mutations cause retinal-specific splicing defects and structural/functional impairments, which are corrected by in situ gene editing.