Vı́ctor de Lorenzo

This study investigates the effects of synthetic horizontal gene transfer in ecological consortia models to promote biodiversity and regulate gene propagation.

The paper develops a theoretical framework to assess the invasion potential of mirror life in ecosystems, showing ecological constraints limit its establishment.

The paper investigates the structural robustness of Escherichia coli TpiA by introducing amino acid insertions and shows that some variants retain wild-type activity despite structural perturbations.

The paper parameterizes five bacterial gene expression systems using a standardized vector format to compare their performance across different inducers.

The paper introduces a version control system for cell engineering that combines cloud-based software with molecular barcoding to enhance transparency and traceability.

This study demonstrates how ssDNA recombineering in bacteria enables hyper-diversification of nanobodies, resulting in variants that recognize new antigens while retaining original specificity.

The paper demonstrates the use of Targetron technology with CRISPR-aided counterselection to efficiently deliver exogenous DNA sequences into Pseudomonas putida.

The paper standardizes an inducible expression system for bacterial hosts by refactoring the AlkS/PalkB module to improve predictability and interoperability.

This paper introduces a method called 3D-seq to map DNA-protein interaction sites in vivo using a modified cytosine deaminase, demonstrating its application in Pseudomonas aeruginosa transcription factors.

The paper characterizes 20 genetic NOT inverters across 7 bacterial contexts, showing context-dependent behaviors that enhance circuit compatibility and interoperability in synthetic biology.

The paper describes a broad host range inverter package for genetic circuit design in Gram-negative bacteria, showing its functionality in Pseudomonas putida and portability across species.

The paper describes the display of designer scaffoldin proteins on Pseudomonas putida strains to enable extracellular enzyme activity for biotechnological applications.

The paper describes the creation of a surface-naked Pseudomonas putida strain by deleting genes encoding outer-facing structures, enabling controlled display of artificial adhesins and analysis of bacterial interactions.

The paper introduces a digital twinning system to link engineered cells to their digital history, enabling version control for collaborative strain engineering.

The paper examines features of a cyanobacterial regulatory node for optogenetic device interoperability between E. coli and P. putida, identifying translational control as a bottleneck and proposing optimization strategies.

The paper designs a digitalizer module to suppress basal transcription in Gram-negative bacteria, enabling all-or-nothing gene expression, and validates it in E. coli and Pseudomonas putida.

The paper identifies an amino acid critical for inducer recognition in bacterial regulators and creates engineered TFs responsive to aspirin.

The paper introduces FENIX, a system for post-translational control of protein levels in E. coli, enabling uncoupling of bacterial growth from biopolymer production.

This paper modifies Pseudomonas putida EM42 to co-utilize disaccharides, pentoses, and hexoses by introducing genetic modules for cellobiose and xylose metabolism.