Jonathan M. Conway

Development of a CRISPR-Cas9 genome editing system for the extremely thermophilic bacterium Anaerocellum bescii

This paper develops a CRISPR-Cas9 system for Anaerocellum bescii, enabling gene deletions and vector maintenance through thermostable Cas9.

Genetic determinants of Brevundimonas-mediated attenuation of plant pattern-triggered immunity

This study identifies genetic determinants of Brevundimonas-mediated attenuation of plant pattern-triggered immunity, finding TonB1 as the key factor.

Structural insights into xyloglucan recognition by an ABC transporter from a Gram-positive, thermophilic bacterium

The paper identifies an ABC transporter for xyloglucan uptake in Anaerocellum bescii and solves its substrate-binding protein structure, revealing a unique mode of xyloglucan recognition.

Expanding the Promoter Toolbox for Metabolic Engineering in the Lignocellulolytic Thermophile Anaerocellum bescii

This study identifies and characterizes new promoters in Anaerocellum bescii to enhance metabolic engineering capabilities for ethanol production.

A Highly Conserved ABC Transporter Mediates Cello-Oligosaccharide Uptake in the Extremely Thermophilic, Lignocellulolytic Bacterium Anaerocellum (f. Caldicellulosiruptor) bescii

This study identifies an ABC transporter in Anaerocellum bescii responsible for cello-oligosaccharide uptake, showing genetic deletion impairs growth on cellulose and cellobiose.

Engineering auxin degradation into root-associated bacteria promotes plant growth

The paper engineers auxin-degrading bacteria to alleviate root growth inhibition and improve plant growth.

Functional and structural characterization of AtAbf43C: An exo-1,5-α-L-arabinofuranosidase from Acetivibrio thermocellus DSM1313

This paper characterizes the function and structure of AtAbf43C, an exo-1,5-α-L-arabinofuranosidase from A. thermocellus, showing it acts on arabino-oligosaccharides with a specific binding pocket and catalytic triad.

A Straightforward and Robust Enzymatic Reporter System for Anaerobic Thermophiles

This paper develops a robust enzymatic reporter system for anaerobic thermophiles to overcome background activity and enable reliable protein expression assessment.

Maltodextrin Transport in the Extremely Thermophilic, Lignocellulose Degrading Bacterium Anaerocellum bescii (f. Caldicellulosiruptor bescii)

This paper identifies and characterizes two maltodextrin ABC transporters in Anaerocellum bescii, revealing their substrate specificity and biophysical properties to enhance understanding of oligosaccharide transport in thermophilic bacteria.

A type II secreted subtilase from commensal rhizobacteria disarms the immune elicitor peptide flg22

The paper identifies a type II secreted subtilase from commensal bacteria that cleaves the immune elicitor flg22, suppressing plant immune responses.