This study identifies nucleoporins and EPS-1 as factors anchoring germ granules to NPCs in C. elegans, revealing their role in balancing piRNA and RNAi pathways.

8 citations

C. elegansexperimental assay

𝕏 PostBluesky

Molecular BiologybioRxivPreprintFeb 14, 2025

Analysis of crosslinking sites suggests C. elegnas PIWI Argonaute exhibits flexible conformations for target recognition

Wei Wu, Dong-En Lee, Chi-Jung Chung…·Heng-Chi Lee

The paper develops MUTACLASH to analyze CIMs in CLASH data, revealing that CIMs are enriched at piRNA/miRNA target sites and suggest flexible conformations of PRG-1 for target recognition.

1 citation

C. elegansChIP-seq

𝕏 PostBluesky

GeneticsGeneticsPeer-reviewedMay 20, 2023

Sensitized piRNA reporter identifies multiple RNA processing factors involved in piRNA-mediated gene silencing

Jordan S. Brown, Donglei Zhang, Olivia J. Gaylord…·Heng-Chi Lee

9 citations

C. elegansexperimental assay

𝕏 PostBluesky

GenomicsbioRxivPreprintJun 8, 2022

Transcriptome-wide analysis suggests piRNAs preferentially recognize the coding region of mRNAs in C. elegans

Wei-Sheng Wu, Jordan S. Brown, Sheng-Cian Shiue…·Heng-Chi Lee

The paper identifies that C. elegans piRNAs preferentially bind to coding regions of mRNAs, leading to secondary silencing small RNAs, and shows CSR-1 inhibits this process through two mechanisms.

2 citations

C. elegansbulk RNA-seq

𝕏 PostBluesky