Author

Raphael Bueno

Brigham and Women's HospitalORCID 0000-0002-3231-5491Unclaimed ORCID profile · follow to get notified of new preprints

Biology Disciplines

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Signature Assays, Organisms & Methods

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Approach:experimental assay· 2 Organism:human· 2 Assay:scRNA-seq· 2 Approach:statistical model· 2 Research Focus:benchmarking· 1 Assay:bulk RNA-seq· 1 Research Focus:cell type annotation· 1 Research Focus:cell-cell communication· 1

Frequent Co-Authors

Aaron N. HataiD· 1 shared Ahmed A. Sadek· 1 shared Alex K. ShalekiD· 1 shared Alexander M. TsankoviD· 1 shared Anand G. PateliD· 1 shared Åsa KarlströmiD· 1 shared Asaf RotemiD· 1 shared Assunta De RienzoiD· 1 shared

Activity by Year

2020-2025 · peak 1/yr

Co-Author Collaboration Network

10 top collaborators · 24 mutual co-author links

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1 shared paperGenomics

Aaron N. HataORCID

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2 papers

Recent·Most cited

Comprehensive multi-site profiling of the malignant pleural mesothelioma micro-environment identifies candidate molecular determinants of histopathologic type

David T. Severson, Samuel T. Freyaldenhoven, Benjamin J. Wadowski, Yin Pun Hung, Travis K. Hughes, Beow Yong Yeap, William G. Richards, Sanna Laaksonen, Lucian R. Chirieac, Matthew Brian Couger, Roderick V. Jensen, Ahmed A. Sadek, Ilkka Ilonen, Kimberly Vermilya, Simona Innocenti, , Raphael Bueno

This study uses single-cell RNA-sequencing and other omics data to analyze the molecular features of pleural mesothelioma subtypes and identify potential drivers of tumor cell states.

Journal of Thoracic Oncology · Nov 28, 2025Peer-reviewedCancer Biology

A single-cell and single-nucleus RNA-seq toolbox for fresh and frozen human tumors

Michal Slyper, Caroline B. M. Porter, Orr Ashenberg, Julia Waldman, Eugene Drokhlyansky, Isaac Wakiro, Christopher S. Smillie, Gabriela Smith-Rosario, Jingyi Wu, Danielle Ashley Dionne, Sébastien Vigneau, Judit Jané‐Valbuena, Timothy L. Tickle, Sara Napolitano, Mei-Ju Su, , Aviv Regev

The paper develops a toolbox for scRNA-Seq and snRNA-Seq of fresh and frozen human tumors, testing it across eight tumor types and optimizing protocols for different tissue characteristics.

Nature Medicine · May 1, 2020Peer-reviewedGenomics