Author

Sekhar Kambakam

Animal and Plant Health Inspection ServiceORCID 0000-0002-3331-3754Unclaimed ORCID profile · follow to get notified of new preprints

Biology Disciplines

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Microbiology· 2 Genetics· 1

Signature Assays, Organisms & Methods

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Approach:experimental assay· 3 Research Focus:variant calling· 2 Research Focus:benchmarking· 1 Research Focus:cell type annotation· 1 Research Focus:database / resource· 1 Assay:DNA methylation· 1 Research Focus:gene regulatory network inference· 1 Research Focus:host-pathogen & immune response· 1

Frequent Co-Authors

Julia Thomas· 2 shared Rachel M. PalinskiiD· 2 shared Suelee Robbe‐AustermaniD· 2 shared Fang LiuiD· 1 shared Jeffrey J. EssneriD· 1 shared Jordan M. WelkeriD· 1 shared Karl J. ClarkiD· 1 shared Karthik K. Shanmuganatham· 1 shared

Activity by Year

2022-2026 · peak 2/yr

Co-Author Collaboration Network

10 top collaborators · 21 mutual co-author links

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2 shared papersMicrobiology

Julia Thomas

Mutual co-authors in cluster

Author profile →

3 papers

Recent·Most cited

Confirmation of gamma irradiation mediated inactivation of a Vero adapted African swine fever virus Lisbon 60 strain for molecular assays

Sekhar Kambakam, Julia Thomas, Tod P. Stuber, Ping Wu, Suelee Robbe‐Austerman, Rachel M. Palinski

This study confirms that gamma irradiation at 50 kGy fully inactivates ASFV while preserving nucleic acid integrity for molecular assays.

Frontiers in Microbiology · Sep 29, 2026Peer-reviewedMicrobiology

Rapid identification of African swine fever virus in diagnostic samples using CRISPR-Cas

Sekhar Kambakam, Julia Thomas, Suelee Robbe‐Austerman, Karthik K. Shanmuganatham, Rachel M. Palinski

This paper develops a CRISPR-Cas12a assay for rapid, sensitive, and specific detection of African Swine Fever Virus in diagnostic samples, achieving high sensitivity and specificity comparable to qPCR.

Journal of Veterinary Diagnostic Investigation · Mar 25, 2026Peer-reviewedMicrobiology

Zebrafish Cre/lox regulated UFlip alleles generated by CRISPR/Cas targeted integration provide cell-type specific conditional gene inactivation

Maira P. Almeida, Sekhar Kambakam, Fang Liu, Zhitao Ming, Jordan M. Welker, Wesley A. Wierson, Laura E. Schultz‐Rogers, Stephen Carl Ekker, Karl J. Clark, Jeffrey J. Essner, Maura A. McGrail

The paper develops a CRISPR/Cas9-based method to generate Cre/lox regulated conditional gene alleles in zebrafish, enabling cell-type specific gene inactivation.

eLife · Jun 17, 2022Peer-reviewedGenetics