Stephen Carl Ekker

The paper generates a systematic in vivo atlas of loss-of-function alleles in zebrafish mitochondrial genes, revealing their impact on bioenergetics and hair cell function.

This study creates a zebrafish Runx1 lineage tracing tool to track hematopoietic development and adult blood cell lineages.

This paper describes a persistent AI scientist system that operates continuously, improves through memory and feedback, and generates hypotheses and scientific outputs over time.

This paper develops a non-destructive method for genotyping zebrafish larvae for mitochondrial and nuclear DNA, validated through PCR and sequencing, and shows its utility for gene editing studies.

This study investigates the protective role of VEGF-B/NRP1 signaling in cardiomyocytes after myocardial infarction, showing improved mitochondrial function and cardiac regeneration.

This paper develops zebrafish hand2 Cre and CreERT2 drivers using CRISPR-Cas9 to label mesodermal and ectodermal lineages, revealing lineage contributions to various tissues and vasculature.

This paper introduces DdCBEs with modified TALE proteins that remove the 5-T constraint, enabling efficient and specific mitochondrial base editing at previously inaccessible sites.

This paper presents a zebrafish model of Leigh Syndrome, demonstrating liver dysfunction's critical role in mitochondrial disease through genetic rescue.

This study identifies Dnajb6 as a gene causing sick sinus syndrome through a phenotype-based genetic screen in zebrafish.

The paper presents a collection of zebrafish genetic mutants for studying mitochondrial biology and disease.

The paper develops a CRISPR/Cas9-based method to generate Cre/lox regulated conditional gene alleles in zebrafish, enabling cell-type specific gene inactivation.

The paper introduces FusXTBE, a TALE base editor for editing mtDNA in human cells and zebrafish, achieving high editing efficiency and precision.

This paper introduces a Donorguide technology that improves HDR efficiency and precision by fusing an ssDNA template to tracrRNA, showing enhanced results in vitro and in vivo.

This paper generates zebrafish Cre drivers by integrating a 2A-Cre transgene into proneural genes, resulting in functional Cre expression in specific cell populations.

The paper introduces MENTHU, a tool predicting MMEJ-repaired genotypes in zebrafish, showing improved sensitivity over inDelphi and validating its use for gene editing applications.

This study shows that VEGF signaling is activated by UV exposure and that inhibiting it can prevent sunburn symptoms in mice, suggesting a new therapeutic approach for UV-induced skin damage.

The paper identifies differences in hair cell biology and behavior in zebrafish myo7aa-/- mutants and shows that L-type voltage-gated calcium channel agonists can rescue these phenotypes.

This paper characterizes ErCas12a, a new CRISPR/Cas system, showing it enables efficient DNA repair and gene integration in zebrafish and human cells.

GeneWeld enables efficient and precise targeted integration in multiple species using CRISPR/Cas9 with minimal homology arms.

The paper describes a zebrafish gene break transposon library for genomic discovery and disease modeling, identifying novel gene expression patterns and generating models for human disease loci.

The paper introduces LipoGlo, a system using ApoB-fused luciferase to monitor lipoprotein atherogenicity in zebrafish, revealing new localization patterns and identifying a regulator of lipoprotein size.

This study identifies RXRA as a therapeutic target for doxorubicin-induced cardiomyopathy in zebrafish by showing its endothelial-specific function and response to FDA-approved agonists.

This study shows that activating integrins and using BDNF together can promote axon regeneration in the spinal cord by overcoming inhibitory factors like MAG.

This paper demonstrates that inducing Microhomology-mediated End Joining (MMEJ) in vivo can generate highly homogeneous mutant alleles, with a novel algorithm provided for predicting MMEJ-inducing sites.

This paper introduces a method to engineer precise deletions in the mitochondrial genome using TALE proteins in zebrafish and human cells.