Karl J. Clark

The paper generates a systematic in vivo atlas of loss-of-function alleles in zebrafish mitochondrial genes, revealing their impact on bioenergetics and hair cell function.

This study creates a zebrafish Runx1 lineage tracing tool to track hematopoietic development and adult blood cell lineages.

This paper develops zebrafish hand2 Cre and CreERT2 drivers using CRISPR-Cas9 to label mesodermal and ectodermal lineages, revealing lineage contributions to various tissues and vasculature.

This paper introduces DdCBEs with modified TALE proteins that remove the 5-T constraint, enabling efficient and specific mitochondrial base editing at previously inaccessible sites.

This paper presents a zebrafish model of Leigh Syndrome, demonstrating liver dysfunction's critical role in mitochondrial disease through genetic rescue.

This study identifies Dnajb6 as a gene causing sick sinus syndrome through a phenotype-based genetic screen in zebrafish.

The paper presents a collection of zebrafish genetic mutants for studying mitochondrial biology and disease.

The paper develops a CRISPR/Cas9-based method to generate Cre/lox regulated conditional gene alleles in zebrafish, enabling cell-type specific gene inactivation.

This study investigates the role of the HPA axis in locomotor adaptation during photoadaptation in zebrafish, finding that it is necessary for rapid adaptation to short light exposures but not for longer exposures.

The paper introduces FusXTBE, a TALE base editor for editing mtDNA in human cells and zebrafish, achieving high editing efficiency and precision.

This paper introduces a Donorguide technology that improves HDR efficiency and precision by fusing an ssDNA template to tracrRNA, showing enhanced results in vitro and in vivo.

This paper generates zebrafish Cre drivers by integrating a 2A-Cre transgene into proneural genes, resulting in functional Cre expression in specific cell populations.

This paper characterizes ErCas12a, a new CRISPR/Cas system, showing it enables efficient DNA repair and gene integration in zebrafish and human cells.

GeneWeld enables efficient and precise targeted integration in multiple species using CRISPR/Cas9 with minimal homology arms.

The paper describes a zebrafish gene break transposon library for genomic discovery and disease modeling, identifying novel gene expression patterns and generating models for human disease loci.

This study shows glucocorticoids inhibit spinal cord regeneration by directly affecting ependymal glia, contrasting with zebrafish regeneration mechanisms.

This paper demonstrates that inducing Microhomology-mediated End Joining (MMEJ) in vivo can generate highly homogeneous mutant alleles, with a novel algorithm provided for predicting MMEJ-inducing sites.

The paper shows that zebrafish locomotor response to acute stressors requires HPI axis activation via mc2r and nr3c1, but not nr3c2.

This paper introduces a method to engineer precise deletions in the mitochondrial genome using TALE proteins in zebrafish and human cells.