Pablo Smircich

This study uses single-cell RNA sequencing to show that T. cruzi trypomastigote cells express trans-sialidase-like superfamily genes with high heterogeneity and no recurrent combinations.

The paper develops an expression-based annotation strategy to identify and quantify small vault RNAs (svtRNAs) in human cells, showing they are abundant and reproducibly detected across datasets.

The paper identifies trans-splicing acceptor sites in TriTryps and shows similar chromatin organization across genes, with histone-containing complexes protecting these sites.

The paper develops a protein structure-based homology search pipeline to annotate kinetoplastid proteins, identifying 23,000 hypothetical proteins and uncovering previously undetected eukaryotic proteins.

The paper investigates the transcriptomic changes in Trypanosoma cruzi epimastigotes with TcUBP1mut knockdown, identifying 276 up- and 426 downregulated genes compared to wildtype.

The paper shows that quantizing nanopore quality scores has minimal impact on downstream analysis and suggests using lossy compression for storage savings.

RENANO improves nanopore FASTQ compression by 39.8% for base call sequences using reference-based methods, with overall improvements of 12.7% in some scenarios.