High-throughput and high-efficiency sample preparation for single-cell proteomics using a nested nanowell chip
Jongmin Jacob Woo, Sarah Williams, Lye Meng Markillie, Song Feng, Chia‐Feng Tsai, Victor Aguilera-Vazquez, Ryan L. Sontag, Ronald Jay Moore, Dehong Hu, Hardeep S. Mehta, Joshua Cantlon-Bruce, Tao Liu, Joshua Adkins, Richard D. Smith, Gérémy Clair, , Ying Zhu
This paper introduces a nested nanowell chip to improve single-cell proteomics throughput and robustness, demonstrating the ability to quantify ~1500 proteins from ~100 cells with low abundance variation.
Nature Communications · Oct 29, 2021Peer-reviewedSystems Biology