Alejandro Couce

This study shows mutation-biased adaptation can intensify in large bacterial populations and lead to unpredictable collateral sensitivity to antibiotics.

The paper examines how single-point mutations in rpsL in E. coli affect evolvability towards multi-aminoglycoside resistance, finding some mutations block further adaptation.

The paper uses directed evolution to study how epistasis shapes mutational paths in KPC-2 carbapenemase, revealing divergent phenotypic outcomes despite similar fitness in selective conditions.

This study shows that during long-term bacterial adaptation, fitness effects of mutations become less severe, but pleiotropic effects in new environments become more severe, indicating trade-offs between robustness and fragility.

This study shows that evolutionary rescue in a bacterial mutualism occurs when one strain reverts to metabolic autonomy, allowing the community to survive lethal stresses despite the other strain's sensitivity.

The paper quantifies fitness interactions between expression variants of two metabolic genes across environments and shows these can be explained by a model incorporating catabolic flux, metabolite toxicity, and expression costs.