María‐Eugenia Guazzaroni

Discovery and Functional Characterization of Salinity-Responsive Promoters in Pseudomonas putida KT2440

The paper identifies and characterizes salt-inducible promoters in Pseudomonas putida KT2440 using reporter plasmids and functional assays.

Differences in GenBank and RefSeq annotations may affect genomics data interpretation for Pseudomonas putida KT2440

This study compares GenBank and RefSeq annotations for Pseudomonas putida KT2440, showing 16% of ORFs differ in genomic position and highlighting potential impacts on RNA-seq analysis.

Characterization of a marine bacteria through a novel metabologenomics approach

The paper characterizes a marine bacteria using a metabologenomics approach, linking genomic and metabolomic data to identify a new biosynthetic gene cluster and bioactive compound.

Alternate routes to acetate tolerance lead to varied isoprenol production from mixed carbon sources in Pseudomonas putida

This paper develops acetate-tolerant Pseudomonas putida strains for isoprenol production using mixed carbon sources and identifies molecular mechanisms underlying tolerance and production.

A termination-aware vector design improves heterologous gene expression in Pseudomonas putida

The paper improves heterologous gene expression in Pseudomonas putida by replacing standard terminators with metagenomic ones, enhancing fluorescent signal output.

Mining novel cis-regulatory elements from the emergent host Rhodosporidium toruloides using transcriptomic data

The paper uses transcriptomic data from Rhodosporidium toruloides to identify novel cis-regulatory elements by predicting transcription factor binding motifs under stress and substrate conditions.

Turning the screw: engineering extreme pH resistance in Escherichia coli through combinatorial synthetic operons

This study engineers Escherichia coli to tolerate extreme acidity by combining metagenomic genes in synthetic operons, significantly increasing survival under acidic shock conditions.

Expanding the toolbox of broad host-range transcriptional terminators for Proteobacteria through metagenomics

This paper identifies novel broad host-range transcriptional terminators from metagenomic data for use in Proteobacteria.

Reverse engineering of an aspirin-responsive regulator in bacteria

The paper identifies an amino acid critical for inducer recognition in bacterial regulators and creates engineered TFs responsive to aspirin.

Big trouble with little inserts: boundaries in metagenomic screenings using lacZα based vectors

The paper identifies false positives in metagenomic screenings using lacZα-based vectors due to small DNA insertions within the lacZα gene, highlighting the need to report screening boundaries.