Jingjing Huang

The paper introduces genetically encoded FRET sensors to detect protease activities in plants, demonstrating their use in monitoring metacaspase and Tobacco Etch Virus protease in vivo.

This study identifies 225 redox-active nuclear proteins in Arabidopsis, including GCN5, modified by sulfenylation under oxidative stress, revealing their functional impact on protein interactions.

CysQuant quantifies cysteine oxidation and protein abundance using DDA or DIA-MS, revealing redox changes in Arabidopsis exposed to light.

The paper uses AlphaFold2 predicted structures to analyze cysteine disulfides and metal binding sites in plant proteomes, finding high agreement with experimental structures and evolutionary trends in metal binding.