Frank Van Breusegem

A redox-regulated RCC1-like protein controls catalase activity in Arabidopsis

This study identifies CAIR1 as a redox-regulated protein that enhances catalase activity in Arabidopsis by facilitating its peroxisomal import and subcellular localization.

Functional diversification of UBP6 in plant immunity through N-degron pathway regulation

The paper shows that UBP6 cleavage generates a proteoform that stabilizes NPR1 and enhances plant immunity through the N-degron pathway.

Conditional stability of HY5 through the ATE N-degron pathway regulates environmental responses in Arabidopsis thaliana

Shedding light on plant proteolysis: genetically encoded fluorescent sensors as tools for profiling protease activities

The paper introduces genetically encoded FRET sensors to detect protease activities in plants, demonstrating their use in monitoring metacaspase and Tobacco Etch Virus protease in vivo.

Cytosolic GLUCOSE-6-PHOSPHATE DEHYDROGENASE 5 is a key player in redox homeostasis during oxidative stress and in oxidative stress-triggered activation of the salicylic acid pathway

This study identifies cytosolic G6PD5 as essential for redox homeostasis and oxidative stress-triggered salicylic acid signaling in Arabidopsis.

Antagonistic functions of the two oxidative pentose phosphate pathway dehydrogenases in shaping oxidative stress responses

The paper identifies an antagonistic relationship between two OPPP enzymes, G6PD5 and PGD2, in regulating oxidative stress and SA signaling through metabolic intermediates.

Lack of AtMC1 catalytic activity triggers autoimmunity dependent on NLR stability

This study shows that lack of AtMC1 catalytic activity causes autoimmunity in Arabidopsis, which depends on NLR stability and can be reversed by overexpressing SNIPER1.

The nuclear sulfenome of Arabidopsis: spotlight on histone acetyltransferase GCN5 regulation through functional thiols

This study identifies 225 redox-active nuclear proteins in Arabidopsis, including GCN5, modified by sulfenylation under oxidative stress, revealing their functional impact on protein interactions.

Scywalker: scalable end-to-end data analysis workflow for nanopore single-cell transcriptome sequencing

Scywalker provides a scalable analysis pipeline for nanopore single-cell transcriptome data, showing good correlation with short-read analyses and enabling isoform-level cell-type-specific expression identification.

CysQuant: simultaneous quantification of cysteine oxidation and protein abundance using data dependent or independent acquisition mass spectrometry

CysQuant quantifies cysteine oxidation and protein abundance using DDA or DIA-MS, revealing redox changes in Arabidopsis exposed to light.

Functionally annotating cysteine disulfides and metal binding sites in the plant kingdom using AlphaFold2 predicted structures

The paper uses AlphaFold2 predicted structures to analyze cysteine disulfides and metal binding sites in plant proteomes, finding high agreement with experimental structures and evolutionary trends in metal binding.

The Plant PTM Viewer, a central resource exploring plant protein modifications. From site-seeing to protein function.

The paper introduces the Plant PTM Viewer, a database integrating over 200,000 PTM sites across five plant species to explore protein modifications and their functional implications.

RCD1 Coordinates Chloroplastic and Mitochondrial Electron Transfer through Interaction with ANAC Transcription Factors

The paper shows RCD1 interacts with ANAC transcription factors to regulate chloroplastic and mitochondrial retrograde signaling via ROS-dependent mechanisms.

The Proteolytic Landscape Of An Arabidopsis Separase-Deficient Mutant Reveals Novel Substrates Associated With Plant Development

The paper identifies novel substrates of separase in Arabidopsis by analyzing proteolytic changes in wild-type and separase mutant root tip cells, revealing roles in pH homeostasis, lipid signaling, and proteolytic network interactions.