Jaco van der Torre

This study examines how intercalating dyes affect DNA loop extrusion by SMC proteins, finding they alter extrusion rates and SMC activity, and introduces a dye-free assay.

The paper introduces a modular DNA toolkit for creating long, customizable DNA molecules with user-defined sequences for single-molecule biophysics applications.

The study identifies two CTCF N-terminal motifs that synergistically stall cohesin-mediated DNA loop extrusion through distinct mechanisms.

The paper shows that all eukaryotic SMC complexes extrude DNA asymmetrically, with direction switches observed in cohesin and SMC5/6, linked to NIPBL turnover and stabilized by STAG1.

The paper shows that dense Rap1 arrays block DNA loop extrusion by condensin in yeast, acting as contact insulators and affecting dicentric chromosome resolution.