Stéphane Marcand

This study presents cryo-EM structures of yeast NHEJ complexes, revealing mechanisms for DNA-end protection and ligation without DNA-PKcs.

The paper reveals that Rap1 inhibits Ku's NHEJ function at telomeres by obstructing Ku's inward translocation, preventing promiscuous repair.

The paper shows that dense Rap1 arrays block DNA loop extrusion by condensin in yeast, acting as contact insulators and affecting dicentric chromosome resolution.

This study shows the Rad55-Rad57 complex promotes Rad51 filament stability on UV-induced single-strand DNA gaps, counteracting error-prone polymerases to favor error-free homologous recombination.

The paper introduces a genetic assay to quantify chromosome fusions in yeast and shows a trade-off in telomere protection and NHEJ vulnerability to ionising radiation.

This study shows that Sir3 promotes NHEJ by inhibiting Sae2-dependent resection through physical interaction, independent of its heterochromatin function.