Roman Barth

The paper introduces a modular DNA toolkit for creating long, customizable DNA molecules with user-defined sequences for single-molecule biophysics applications.

The study identifies two CTCF N-terminal motifs that synergistically stall cohesin-mediated DNA loop extrusion through distinct mechanisms.

This study shows cohesin supercoils DNA during loop extrusion, influencing genome architecture through both looping and supercoiling.

The paper measures the twist induced by eukaryotic SMC proteins during DNA loop extrusion and finds all three SMC complexes induce a consistent -0.6 twist per step.

The paper shows that all eukaryotic SMC complexes extrude DNA asymmetrically, with direction switches observed in cohesin and SMC5/6, linked to NIPBL turnover and stabilized by STAG1.

The paper shows that dense Rap1 arrays block DNA loop extrusion by condensin in yeast, acting as contact insulators and affecting dicentric chromosome resolution.

This study demonstrates that Dynamin A can split dumbbell-shaped liposomes, functioning as a one-component division machinery for synthetic cells.