Iain F. Davidson

DNAsight automates AFM image analysis of chromatin organization, revealing protein-specific structural signatures and nucleosome spacing distributions.

This study shows PDS5 proteins regulate genome architecture by stopping loop extrusion and strengthening CTCF boundaries through NIPBL dissociation from cohesin.

The study identifies two CTCF N-terminal motifs that synergistically stall cohesin-mediated DNA loop extrusion through distinct mechanisms.

The paper shows CTCF controls chromatin boundary permeability through a dynamic conformational ensemble, not static occupancy, influencing cohesin capture probabilities.

This study identifies NIPBL and STAG1's roles in cohesin-mediated DNA loop extrusion by analyzing their differential DNA-cohesin interactions and ATP-dependent regulation.

This study shows cohesin supercoils DNA during loop extrusion, influencing genome architecture through both looping and supercoiling.

The paper measures the twist induced by eukaryotic SMC proteins during DNA loop extrusion and finds all three SMC complexes induce a consistent -0.6 twist per step.

The paper shows that all eukaryotic SMC complexes extrude DNA asymmetrically, with direction switches observed in cohesin and SMC5/6, linked to NIPBL turnover and stabilized by STAG1.