João Vitor Puttini Paixão

This study validates a single oocyte biopsy method in bovine by comparing it to whole oocytes, showing high correlation in gene expression despite minor differences.

This paper predicts oocyte competence in bovine using RNA-seq and WGBS from biopsies, cumulus cells, granulosa cells, and polar bodies, identifying transcriptional and epigenetic differences between cleaved but arrested and blastocyst-stage embryos.