José‐Antonio Daròs

Amplification-free CRISPR/Cas13a-based viroid detection in RNA extracts from infected plants

This paper develops an amplification-free CRISPR-Cas13a platform for direct viroid detection in plant RNA extracts, demonstrating its effectiveness for multiple viroid families.

Bioluminescent sentinel plants enable autonomous diagnostics of viral infections

This paper develops bioluminescent plants that detect viral infections through spectral changes, using a protease-responsive circuit triggered by viral proteases.

A modified Cas9 scaffold allows extension of the virus-induced gene editing technology to the large Potyvirus genus

This paper develops a modified Cas9 scaffold for virus-induced gene editing (VIGE) in Potyviruses, enabling efficient gene editing in plants by eliminating stop codons in the Cas9 sgRNA scaffold.

Comparative genomics-driven design of virus-delivered short RNA inserts triggering robust gene silencing

This study designs virus-delivered short RNA inserts (vsRNAi) for gene silencing in plants, showing effectiveness in Nicotiana benthamiana and crops like tomato and scarlet eggplant.

Arabidopsis thaliana Iron Superoxide Dismutase FeSOD1 Protects ARGONAUTE 1 in a Copper-Dependent Manner

The paper identifies FeSOD1 as a Cu-deficiency-induced interactor of AGO1 that protects it from oxidative stress, preserving miRNA-mediated gene silencing.

Crocetin and crocin production in plant and bacteria using a CCD4 enzyme from Nyctanthes arbor-tristis.

This paper reports the identification of NatCCD4.1, an enzyme from Nyctanthes arbor-tristis, and demonstrates its use in microbial and plant systems to produce crocetin dialdehyde and crocins with high yields.

Potato virus X -delivered CRISPR activation programs lead to strong endogenous gene induction and transient metabolic reprogramming in Nicotiana benthamiana.

This paper demonstrates that PVX-delivered CRISPRa systems can induce strong gene activation and metabolic reprogramming in N. benthamiana.

Intron-assisted, viroid-based production of insecticidal circular double-stranded RNA in Escherichia coli

This paper describes a system for producing insecticidal circular dsRNA in E. coli using viroid scaffolds and introns, demonstrating its effectiveness against Western corn rootworm.

Strong and tunable anti-CRISPR/Cas9 activity of AcrIIA4 in plants

This study shows AcrIIA4 strongly inhibits CRISPR/Cas9 and CRISPRa in plants, with tunable control via auxin-regulated fusion.

Cooperative riboregulation in living cells through allosteric programming of toehold activation

The paper designs cooperative riboregulation in E. coli using a hierarchical toehold activation cascade and a de novo sequence design algorithm.