Edward N. Smith

Thermo-Flux converts stoichiometric metabolic models into thermodynamic-stoichiometric models to improve flux predictions through automated mass and charge balancing and integration of Gibbs energy data.

The paper uses hypoxia-activated fluorescent probes to measure oxygen levels in plant cells and tissues, showing their effectiveness in detecting hypoxia in Arabidopsis thaliana.

Modelling 12 alternative photorespiratory pathways predicts the largest carbon-export gains for carbon-fixing designs (TaCo); complete decarboxylation bypasses help mainly by releasing CO2 in chloroplasts, raising carboxylation and recapturing lost CO2.