José Losa

Thermo-Flux converts stoichiometric metabolic models into thermodynamic-stoichiometric models to improve flux predictions through automated mass and charge balancing and integration of Gibbs energy data.

The paper shows that protein agglomerates in E. coli regulate cytoplasmic rheology, with diffusion inversely correlating with amino acid metabolism protein abundance.

This study quantifies how DNA, mRNA, and proteins contribute to cytoplasmic crowding in E. coli by measuring the diffusion of a 40 nm particle under selective depletion conditions.

This paper develops a photo-switchable isocitrate dehydrogenase in yeast to dynamically control citric acid cycle flux using optogenetics.