This study expands a modular cloning platform to enable rapid, customizable construct assembly for MAPPIT assays, demonstrating its application in studying multiple protein-protein interactions.

FEBS Journal · Sep 15, 2026Peer-reviewedSynthetic Biology

Proximity map for p53 unveils a strong link with PML Nuclear Bodies in HEK293 cells

Edith De Bruycker, Giel Vandemoortele, Delphine De Sutter, George D. Moschonas, Sven Eyckerman

The paper uses BioID proximity labeling to show p53 interacts with PML nuclear bodies and SUMOylation proteins in HEK293 cells, linking these interactions to differences in p53 transcriptional activity compared to HCT116 cells.

bioRxiv · Oct 22, 2025 · PreprintSystems Biology

TurboID proximity interactome mapping reveals NR2E3 association with AP-1 and retinal developmental complexes

Edith De Bruycker, Olivier Zwaenepoel, George D. Moschonas, Delphine De Sutter, Eva D’haene, Charlotte Matton, Laure Vandevelde, Julie Van De Velde, Quinten Mahieu, Sara Dufour, Delphi Van Haver, Louis Delhaye, Simon Devos, Frauke Coppieters, Sven Eyckerman, , Elfride B. W. De Baere

This study maps the proximity interactome of NR2E3 and its G56R variant using TurboID, revealing interactions with retinal development complexes and identifying BCOR as enriched in the mutant network.

bioRxiv · Sep 4, 2025 · PreprintSystems Biology

Virotrap Reveals Salmonella SopB as A Ubiquitinated Cargo for Host ESCRT-0

Margaux De Meyer, Annick Verhee, Hanna Grzesik, Delphine De Sutter, Jon Huyghe, Louis Delhaye, Tessa Van de Steene, Igor Fijałkowski, Veronique Jonckheere, Leander Meuris, Mathieu J.M. Bertrand, Petra Van Damme, Virginie Stévenin, Sven Eyckerman

This study identifies SopB-associated host proteins using Virotrap, revealing SopB's interaction with ESCRT-0 and its role in Salmonella's manipulation of host endosomal trafficking.

bioRxiv · Aug 19, 2025 · PreprintMicrobiology