Multiplex genotyping method to validate the multiallelic genome editing outcomes using machine learning-assisted long-read sequencing
Akihiro Kuno, Yoshihisa Ikeda, Shinya Ayabe, Kanako Kato, Kotaro Sakamoto, Sayaka Suzuki, Kento Morimoto, Arata Wakimoto, Natsuki Mikami, Miyuki Ishida, Natsumi Iki, Yuko Hamada, Megumi Takemura, Yoko Daitoku, Yoko Tanimoto, , Seiya Mizuno
This paper introduces DAJIN, a machine learning-assisted long-read sequencing method for comprehensive multiplex genotyping of genome editing outcomes, including intended and unintended mutations.
PLoS Biology · Jan 18, 2022Peer-reviewedGenomics