Atsushi Yoshiki

Dietary phosphorus restriction enables multigenerational breeding of α-Klotho-deficient rats

This study shows that dietary phosphorus restriction allows for the multigenerational breeding of α-Klotho-deficient rats, improving survival and reproductive outcomes.

Universal method for generating knockout mice in multiple genetic backgrounds using zygote electroporation

This paper introduces a universal method for generating knockout mice in multiple inbred strains using zygote electroporation, enabling efficient phenotyping for gene function and disease research.

Multiplex genotyping method to validate the multiallelic genome editing outcomes using machine learning-assisted long-read sequencing

This paper introduces DAJIN, a machine learning-assisted long-read sequencing method for comprehensive multiplex genotyping of genome editing outcomes, including intended and unintended mutations.

A resource of targeted mutant mouse lines for 5,061 genes

The paper reports the generation of over 5,000 new mouse mutant strains for targeted genes, significantly expanding the available genetic resources for biomedical research.

Re-Evaluating One-step Generation of Mice Carrying Conditional Alleles by CRISPR-Cas9-Mediated Genome Editing Technology

The paper re-evaluates the efficiency of a CRISPR-Cas9 method for generating conditional knockout alleles in mice, finding very low success rates and high rates of undesired alleles.