This study develops a high-throughput single-cell proteomics workflow combining nPOP with quantitative hyperplexing, achieving high sensitivity and quantification across multiple cell types.

Analytical Chemistry · Jul 17, 2026Peer-reviewedCell Biology

AM-DMF-SCP-pro: An Ultrafast and Streamlined Nanolitre-Scale Lab-on-a-Chip Platform for Single-Cell Proteomics and Tumor Microenvironment Profiling

Kai Jin, Zhicheng Yang, Anqi Ding, Rong Wang, Yifan Ma, Yue Wang, Minchu Tang, Yong Wu, Qian Liu, Jiajian Ji, Chunyu Chang, Hu S, Wenfei Dong, Jian Ding, Yuehua Chen, , Hu Zhou

AM-DMF-SCP-pro enables rapid, low-background single-cell proteome profiling in complex samples by streamlining sample preparation and reducing background interference.

bioRxiv · May 4, 2026 · PreprintSystems Biology

Simple One-step Vial-based Pretreatment for Deep Single-cell Proteomics and Its Application to Oocyte Aging

Hui Zhang, Hailu Zhang, Chuanxi Huang, Qing Zeng, Chunyan Tian, Jing Yang, Fu-Chu He, Yun Yang

This paper introduces a simple one-step vial-based pretreatment method for deep single-cell proteomics and applies it to study oocyte aging, identifying age-related protein markers.

JACS Au · May 1, 2025Peer-reviewedBiochemistry