Diego Calderon

The paper identifies active human neural enhancers and CRISPRa gRNAs that upregulate NDD risk genes, providing a resource for cis-regulatory therapy development.

This study profiles 5.3 million cells from 55 rhesus macaques across their lifespan to map age-related changes in brain cell types and identify molecular signatures linked to aging and Alzheimer's disease.

The paper describes a CRISPRa screening method combining multiplex perturbations with sc-RNA-seq to identify cell-type-specific regulatory elements and their target genes.

This paper identifies epigenetic features affecting prime editing efficiency and shows how chromatin state can be manipulated to enhance or reduce editing efficiency at specific genomic loci.

This paper introduces prime-SGE, a prime editing framework for assessing drug resistance variants across multiple oncogenes in a single experiment.

This paper develops scQers, single-cell quantitative expression reporters, to measure enhancer activity across multicellular systems with high precision and range.

This paper reconstructs cellular trajectories of mammalian embryogenesis using scRNA-seq data and identifies key transcription factors at branch points.

TransMPRA combines CRISPR perturbation and MPRAs to identify trans-acting factors influencing enhancer activity.

The paper maps stimulation-responsive chromatin changes in immune cells and identifies variants linked to autoimmunity.

The paper examines regulatory variation in iPSCs and differentiated cells, finding lower individual variation in iPSCs and identifying shared chromatin regions for some cell type-specific QTLs.

RolyPoly links GWAS summary statistics with single-cell RNA-seq to identify trait-relevant cell types and genes, finding associations between microglia and Alzheimer's disease, and oligodendrocytes and schizophrenia.