Yin Cai

The paper develops a framework to create a dynamic protein atlas of human cell division by integrating 4D imaging data with computational modeling.

The paper describes a protocol to generate and validate homozygous fluorescent knock-in cell lines using CRISPR/Cas9 and provides validation methods to ensure accuracy and functionality.

This paper presents a workflow combining FCS-calibrated imaging and 4D microscopy to quantitatively map protein concentrations and copy numbers in live cells.