Nike Walther

Distinct Sox9 single-molecule dynamics characterize adult differentiation and fetal-like reprogrammed states in intestinal organoids

This study uses single-molecule tracking to show Sox9 dynamics change during intestinal organoid differentiation and fetal-like reprogramming, with overexpression causing morphological and functional shifts.

Automated live-cell single-molecule tracking in enteroid monolayers reveals transcription factor dynamics probing lineage-determining function

The paper uses automated live-cell single-molecule tracking to study transcription factor dynamics in mouse enteroid monolayers, revealing contrasting diffusive behaviors that correlate with differentiation stages.

Transgene Expression in Cultured Cells Using Unpurified Recombinant Adeno-Associated Viral Vectors

The paper demonstrates the use of unpurified recombinant adeno-associated viral vectors for transducing cultured cells, showing effectiveness in three previously unreported cell types.

Experimental and computational framework for a dynamic protein atlas of human cell division

The paper develops a framework to create a dynamic protein atlas of human cell division by integrating 4D imaging data with computational modeling.

Generation and validation of homozygous fluorescent knock-in cells using genome editing

The paper describes a protocol to generate and validate homozygous fluorescent knock-in cell lines using CRISPR/Cas9 and provides validation methods to ensure accuracy and functionality.

Quantitative mapping of fluorescently tagged cellular proteins using FCS-calibrated four dimensional imaging

This paper presents a workflow combining FCS-calibrated imaging and 4D microscopy to quantitatively map protein concentrations and copy numbers in live cells.

CTCF, WAPL and PDS5 proteins control the formation of TADs and loops by cohesin

This study demonstrates that CTCF, WAPL, and PDS5 proteins regulate TADs and loops by controlling cohesin's interaction with chromatin.