Christopher P. Johnstone

The paper shows that gene syntax influences supercoiling-mediated transcriptional feedback, affecting adjacent gene expression in human cells.

The paper designs and characterizes ComMAND, a microRNA-based gene circuit that precisely controls transgene expression in diverse primary cells.

The paper measures mRNA and protein levels in single cells for synthetic promoters, finding that promoter strength affects both transcription and translation, and introduces a framework for comparing gene regulation.

The paper identifies a quiescent-like state with reduced nuclear size and transcriptional activity that enables efficient TF-mediated conversion of fibroblasts to neurons.

This study models how supercoiling-mediated feedback couples gene expression in synthetic and native systems, showing it influences expression profiles, variance, and regulatory interactions.