Deon S. Ploessl

This paper demonstrates that self-amplifying RNA (saRNA) enables durable, integration-free transgene expression in hiPSCs, supporting cell-fate programming and functional reporter analysis.

This paper develops a dual-fluorescent sensing platform to measure intracellular redox state and extracellular respiration in yeast, revealing distinct oxidative stress responses between Saccharomyces cerevisiae and Yarrowia lipolytica.

STRAIGHT-IN Dual enables efficient, single-copy integration of two DNA payloads into hiPSCs, improving genetic engineering for synthetic biology applications.

The paper shows that gene syntax influences supercoiling-mediated transcriptional feedback, affecting adjacent gene expression in human cells.

This paper introduces DASIT, a protein-based circuit for antigen-specific cell selection, demonstrating its utility in genome engineering, CRISPR screening, and cell therapy manufacturing.

DIAL is a framework for designing synthetic promoters that generate heritable, tunable gene expression setpoints across cell types.

The paper measures mRNA and protein levels in single cells for synthetic promoters, finding that promoter strength affects both transcription and translation, and introduces a framework for comparing gene regulation.