Author

Sierra K. Lear

Gladstone InstitutesORCID 0000-0002-7410-9688Unclaimed ORCID profile · follow to get notified of new preprints

Biology Disciplines

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Signature Assays, Organisms & Methods

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Approach:experimental assay· 3 Organism:bacteria (other)· 1 Research Focus:benchmarking· 1 Assay:DNA methylation· 1 Research Focus:drug response / drug discovery· 1 Research Focus:gene regulatory network inference· 1 Organism:human· 1 Research Focus:protein design· 1

Frequent Co-Authors

Seth L. ShipmaniD· 3 shared Santi Bhattarai-KlineiD· 2 shared Santiago C. LopeziD· 2 shared Chloe B. FishmaniD· 1 shared Elana R. LockshiniD· 1 shared George McDonald ChurchiD· 1 shared Jeff NivalaiD· 1 shared Jose A. Nunez· 1 shared

Activity by Year

2021-2023 · peak 1/yr

Co-Author Collaboration Network

10 top collaborators · 32 mutual co-author links

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3 shared papersSynthetic Biology

Seth L. ShipmanORCID

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3 papers

Recent·Most cited

High-throughput colocalization pipeline quantifies efficacy of mitochondrial targeting signals across different protein types

Sierra K. Lear, Jose A. Nunez, Seth L. Shipman

The paper develops a high-throughput pipeline to assess mitochondrial targeting signal efficacy across different protein types by quantifying colocalization.

bioRxiv · Apr 3, 2023 · PreprintSynthetic Biology

Recording gene expression order in DNA by CRISPR addition of retron barcodes

Santi Bhattarai-Kline, Sierra K. Lear, Chloe B. Fishman, Santiago C. Lopez, Elana R. Lockshin, Max G. Schubert, Jeff Nivala, George McDonald Church, Seth L. Shipman

This paper introduces a method to record transcriptional event timing in living genomes using CRISPR-integrated retron barcodes.

Nature · Jul 27, 2022Peer-reviewedSynthetic Biology

Improved architectures for flexible DNA production using retrons across kingdoms of life

Santiago C. Lopez, Kate D Crawford, Sierra K. Lear, Santi Bhattarai-Kline, Seth L. Shipman

The paper improves retron architectures to enhance DNA production across different organisms, leading to more efficient genome editing.

Nature Chemical Biology · Dec 23, 2021Peer-reviewedSynthetic Biology