This study develops a plasma protein binding model to predict unbound cortisol and cortisone concentrations by accounting for changes in ligand and protein concentrations across physiological states.

bioRxiv · Apr 17, 2026 · PreprintPharmacology & Toxicology

Differential retinoic acid responses across testicular development in vitro

Brad C Hansen, Sarah A Hatem, Lucas Huang, John Kenneth Amory, Elaine M. Faustman, Edward J. Kelly

The paper examines how retinoic acid responses differ during testicular development in vitro, showing dose-dependent Stra8 activation and media effects on spermatogonial markers.

bioRxiv · Dec 31, 2025 · PreprintPharmacology & Toxicology

Development and Validation of a Novel LC-MS/MS Based Proteomics Method for Quantitation of Retinol Binding Protein 4 (RBP4) and Transthyretin (TTR)

Aprajita S. Yadav, Katya B. Rubinow, Alex Zelter, Aurora K. Authement, Bryan R. Kestenbaum, John Kenneth Amory, Nina Isoherranen

This study develops and validates an LC-MS/MS method for absolute quantitation of RBP4 and TTR in serum and plasma, showing good linearity, precision, and correlation with ELISA.

Journal of Proteome Research · Sep 10, 2025Peer-reviewedPhysiology

Open-Channel Droplet Microfluidic Platform for Passive Generation of Human Sperm Microdroplets

Tristan M. Nicholson, Jodie C. Tokihiro, Wan‐chen Tu, Jian Wei Khor, Ulri N. Lee, Erwin Berthier, John Kenneth Amory, Thomas J. Walsh, CHARLES H. MULLER, Ashleigh B. Theberge

The paper presents a microfluidic platform that passively generates sub-microliter volumes of purified human sperm for vitrification, preserving sperm kinematics.

ACS Biomaterials Science & Engineering · Feb 12, 2025Peer-reviewedBioengineering