This study identifies HTLV-1 splice sites in gene vectors causing splicing perturbations in transgenic human cells and shows that modifying these sites can enhance genomic safety without compromising therapeutic efficacy.

bioRxiv · Jan 28, 2023 · PreprintGenomics

HMGXB4 Targets Sleeping Beauty Transposition to Vertebrate Germinal Stem Cells

Anantharam Devaraj, Manvendra Singh, Suneel A. Narayanavari, Guo Yong, Jiaxuan Wang, Jichang Wang, Mareike Becker, Oliver Walisko, Andrea J. Schorn, Zoltán Cseresznyés, Dawid P. Grzela, Tamás Raskó, Matthias Selbach, Zoltán Ivics, Zsuzsanna Izsvák

This study identifies HMGXB4 as a regulator of Sleeping Beauty transposition in vertebrate germinal stem cells, linking it to chromatin dynamics and stemness maintenance during early development.

bioRxiv · Jun 15, 2020 · PreprintDevelopmental Biology

Mutant screen for reproduction unveils depression-associated Piccolo's control over reproductive behavior

Gerardo A. Medrano, Manvendra Singh, Erik J. Plautz, Levi B. Good, Karen M. Chapman, Jaideep Chaudhary, Priscilla Jaichander, Heather M. Powell, Ashutosh Pudasaini, John Michael Shelton, James A. Richardson, Xian-Jin Xie, Zoltán Ivics, Christine Braun, Frauke Ackermann, , F. Kent Hamra

The paper identifies Pclo mutations in rats that affect reproductive behavior and link to depression-associated genes.

bioRxiv · Mar 19, 2020 · PreprintGenetics

RNA-guided Retargeting of Sleeping Beauty Transposition in Human Cells

Adrian Kovač, Csaba Miskey, Michael Menzel, Esther Grueso, Andreas Gogol‐Döring, Zoltán Ivics

This paper demonstrates CRISPR/Cas9-guided targeting of Sleeping Beauty transposition in human cells to achieve specific and efficient gene insertion.

eLife · Mar 6, 2020Peer-reviewedMolecular Biology