Re-purposing Ac/Ds transgenic system for CRISPR/dCas9 modulation of enhancers and non-coding RNAs in zebrafish
Vanessa Chong-Morrison, Sarah Mayes, Filipa Costa Simões, Upeka Senanayake, Dervla S. Carroll, Paul Richard Riley, Stephen W. Wilson, Tatjana Sauka‐Spengler
The paper adapts the Ac/Ds transposition system to enable CRISPR/dCas9 interference for studying non-coding RNAs and enhancers in zebrafish without altering genetic sequences.
Biology Open · Jun 15, 2023Peer-reviewedDevelopmental Biology