This study identifies the absence of lipoprotein biosynthesis machinery in Orientia tsutsugamushi and describes alternative mechanisms for its proteins.

bioRxiv · Oct 21, 2026 · PreprintMicrobiology

Genome scale identification of new genes using saturated reporter transposon mutagenesis

Emily C. A. Goodall, Freya J Hodges, Weine Kok, Budi Permana, Thom Cuddihy, Zihao Yang, Nicole Kahler, Kenneth Shires, Karthik Pullela, Von Vergel L. Torres, Jessica L. Rooke, Antoine Delhaye, Jean‐François Collet, Jack Alfred Bryant, Brian M. Forde, , Ian R. Henderson

The paper identifies new protein-coding sequences in E. coli using transposon mutagenesis and translation reporters, revealing previously unannotated genes.

Nucleic Acids Research · Jul 30, 2025Peer-reviewedMicrobiology

Wild-type and single-O-antigen repeat outer-membrane vesicles induce equivalent protection against homologous and heterologous Salmonella challenge

Areej Alshayea, Siân E. Jossi, Edith Marcial‐Juárez, Marisol Pérez-Toledo, Ruby Persaud, Anna E. Schager, Daniel Nyberg Larsen, Gvantsa Gutishvili, Jamie Pillaye, Fernanda Escobar-Riquelme, Kubra Aksu-Istil, Jack Alfred Bryant, William G. C. Horsnell, Manuel Banzhaf, Jakub Zbigniew Kaczmarek, , Adam F. Cunningham

This study shows that OMVs with different O-antigen lengths induce similar protection against Salmonella infections, and boosting enhances cross-protection.

bioRxiv · Jul 3, 2024 · PreprintImmunology

TraDIS-validate: a method for curating ordered gene-replacement libraries

Emily C. A. Goodall, Faye C. Morris, Samantha A McKeand, Rudi Sullivan, Isabel A. Warner, Emma Sheehan, Gabriela Boelter, Christopher Icke, Adam F. Cunningham, Jeffrey A. Cole, Manuel Banzhaf, Jack Alfred Bryant, Ian R. Henderson

TraDIS-validate is a method to validate ordered gene-replacement libraries, achieving 99.7% accuracy in identifying correct kanamycin cassette positions in the Keio library.

bioRxiv · Feb 16, 2022 · PreprintGenetics