Vladimir E. Shevchik

The paper investigates the structural basis of endopilus assembly, stability, and functional specificity in bacterial type II secretion systems using comparative analysis of two pathogens.

The paper uses AlphaFold and cryo-EM to reveal the structure of the type II secretion system's pilus assembly platform and its organization across membranes.

The paper identifies OutB, an inner membrane protein in Dickeya dadantii, as covalently attached to peptidoglycan, similar to Braun lipoprotein, and shows that Ldt03 and Ldt84 enzymes mediate this attachment through amide bond formation.